Journal: Experimental and Therapeutic Medicine

Article Title: Effects of curcumin on the apoptosis of cardiomyocytes and the expression of NF-κB, PPAR-γ and Bcl-2 in rats with myocardial infarction injury

doi: 10.3892/etm.2016.3858

Figure Lengend Snippet: Optical density of NF-κB and PPAR-γ protein expression in the cardiomyocytes of rats (mean ± standard deviation).

Article Snippet: Following blocking with 10% goat serum at 37°C for 15 min, the slices were incubated with rabbit anti-PPAR-γ polyclonal antibody (Peprotech Inc., Rocky Hill, NJ, USA; 1:200) or rabbit anti-NF-κBp6 polyclonal antibody (Peprotech Inc.; 1:100) at 4°C overnight.

Techniques: Expressing, Control

Journal: Plant Physiology

Article Title: Glutathione Regulates 1-Aminocyclopropane-1-Carboxylate Synthase Transcription via WRKY33 and 1-Aminocyclopropane-1-Carboxylate Oxidase by Modulating Messenger RNA Stability to Induce Ethylene Synthesis during Stress ^{1 [OPEN]}

doi: 10.1104/pp.15.01543

Figure Lengend Snippet: Characterization of T4 generation AtECS lines. Leaves from 4-week-old plants grown in growth chamber were used for analysis. A, Four-week-old Col-0, AtECS1, and AtECS19 plants. B, Genomic DNA PCR screening of γ-ECS and nptII genes in AtECS lines. C, Southern-blot analysis of AtECS lines for the transgene integration. Single copy insertion of the transgene was detected in AtECS1, while two copies were detected in AtECS19. D, qRT-PCR analysis for γ-ECS gene expression in AtECS lines. The relative transcript abundance was found to be more than 2-fold higher in AtECS lines compared with Col-0. E, Western-blot analysis of AtECS lines showing γ-ECS protein overexpression. F, Total GSH content estimation by HPLC analysis. GSH content was found to be 2.24- and 1.92-fold higher in AtECS1 and AtECS19, respectively, compared with Col-0. G, Estimation of GSH:oxydized GSH (GSSG) ratio in AtECS lines. All experiments were repeated three times. FW, Fresh weight; E, EcoRI; H, HindIII.

Article Snippet: The γ-ECS protein bands were detected by using a rabbit polyclonal anti-γ-ECS antibody as the primary antibody and an anti-rabbit IgG conjugated to horseradish peroxidase as the secondary antibody (Agrisera).

Techniques: Southern Blot, Quantitative RT-PCR, Gene Expression, Western Blot, Over Expression

Journal: PLoS Pathogens

Article Title: Nipah virus induces two inclusion body populations: Identification of novel inclusions at the plasma membrane

doi: 10.1371/journal.ppat.1007733

Figure Lengend Snippet: (A, B) Vero76 cells were transfected with NiV N and NiV P eGFP to form IBs (IB peri ). At 24 h p.t. cells were fixed with 4% PFA and permeabilized with methanol/acetone. IBs were detected by P eGFP autofluorescence (IB peri ). Cellular aggresome markers (γ-tubulin and vimentin) were detected with specific antibodies (red). (C, D) NiV N and NiV P eGFP were coexpressed with non-related cytosolic proteins (red). Measles virus matrix protein (measles M) was detected with a specific monoclonal antibody (C) and reporter mCherry was detected by autofluorescence (D). Nuclei were counterstained with DAPI (blue). Only merged confocal images are shown. IBs within the boxed areas are shown in higher magnification. Scale Bars, 10 μm.

Article Snippet: The mouse anti-bromodeoxyuridine (clone BMC9318; 1:20) and rabbit anti-γ-tubulin (T3559; 1:1000) antibodies were supplied by Merck.

Techniques: Transfection

Journal: PLoS Pathogens

Article Title: Nipah virus induces two inclusion body populations: Identification of novel inclusions at the plasma membrane

doi: 10.1371/journal.ppat.1007733

Figure Lengend Snippet: Vero76 cells were infected with NiV or NiVΔM at a MOI of 0.05 or 0.01, respectively. At 18.5 h p.i. cells were fixed with 4% PFA for 48 h and permeabilized then with methanol/acetone. Cells were stained with an anti-N serum to visualize IBs (green) and with a Zenon-labeled anti-M peptide serum (cyan) to identify M-positive IB PM . For γ-tubulin detection, cells were incubated with anti-γ-tubulin mouse antibodies (red). (A) A confocal section through a NiV-induced syncytium is shown. Magnifications and individual staining of the boxed areas are presented in the bottom panel. (B) M-negative and y-tubulin positive IB peri . (C) M-positive and y-tubulin negative IB PM . (D) Colocalization of M-positive IB PM and M-negative IB peri with y-tubulin in NiV-induced syncytia (n = 8) was quantified using the ImageJ-based macro IB-Coloc. Error bars indicate the standard error of the mean. Statistical significance is indicated by asterisks (unpaired t-test; ***, p < 0.001). (E) A confocal section through a NiVΔM-induced syncytium is shown. The right panel shows a magnification and individual staining of the IBs in the boxed area.

Article Snippet: The mouse anti-bromodeoxyuridine (clone BMC9318; 1:20) and rabbit anti-γ-tubulin (T3559; 1:1000) antibodies were supplied by Merck.

Techniques: Infection, Staining, Labeling, Incubation